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Nucleic Acids Research, 1979, Vol. 7, No. 7 2031-2044
© 1979

Articles

Distribution of estradiol receptor and vitellogenin gene in chick liver chromatin fractions

Audrey Alberga, Agnés Tran and Etienne Emile Baulieu

Université Paris-Sud, Département de Chimie Biologjque, Unite de Recherches sur le Métabolisme Moleculaire et la Physio-Pathologie des Stéroides, INSERM 78, Avenue du Gé'neral Leclerc, 94270 Kremlin-Bicêtre, France

Received August 29, 1979. The distribution of estradiol receptor and vitellogenin gene was studied in estradiol stimulated chick liver chromatin fractions prepared by limited DNAse II digestion and MgCl2 precipitation. The receptor was found in all fractions, undigested chroroatin (P1), Mg2+ insoluble chromatin (P2) and Mg soluble chromatin (S2). This last fraction was rich in acidic proteins, had a high protein: DNA ratio (7.0 w/w), contained 28% of rapidly labelled RNA, 20% of the receptor, 3–5% of chromatin DNA and showed a 2 fold enrichment of vitellogenin DNA sequences over unfractionated chromatin as well as P1 and P2 DNA. On isopycnic metrizamide gradients, all chromatin fractions showed a receptor peak banding at 1.23 g/cm3, the density of nucleoproteins. Hybridization experiments showed that the DNA banding at this density in fraction S2 was enriched 4 fold in vitellogenin DNA sequences over unfractionated chro-matin as well as PI and P2 DNA. These results suggest an association of hormone receptor complex with nucleoprotein structures of an apparently active chromatin fraction.


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