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Nucleic Acids Research, 1980, Vol. 8, No. 1 153-168
© 1980

Articles

Evidence for the role of double-helical structures in the maturation of Simian Virus-40 messenger RNA

Nancy H. Chiu, Walter B. Bruszewski and Norman P. Salzman

Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, MD 20205, USA

Received October 22, 1979. Simian Virus-40 infected BSC-1 cells were pretreated with glucosamine and briefly pulsed with [3H]-uridine. The labeling can be halted instantaneously by the addition of cold uridine and glucosamine. Under these pulse-chase conditions, the inhibitory effects of the intercalating agent proflavine on the processing of prelabeled nuclear RNA precursors were examined in vivo. Proflavine inhibits the cleavage of viral nuclear RNA precursors. However, turnover of the mature viral mRNAs in the cytoplasm is not inhibited. The effect of proflavine on processing is not a secondary consequence of its inhibition of protein synthesis. The data suggest that base-paired secondary structures in the primary transcripts are important processing signals in the generation of viral mRNA molecules.


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