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Nucleic Acids Research, 1980, Vol. 8, No. 10 2133-2146
© 1980

Articles

Identification of recombinant phages containing sequences from different rat myosin heavy chain genes

U. Nudel*, D. Katcoff*, Y. Carmon*, D. Zevin-Sonkin*, Z. Levi*, Y. Shaul{dagger}, M. Shani* and D. Yaffe*

*Departments of Cell Biology, The Weizmann Institute of Science Rehovot, Israel {dagger}Departments of Virology, The Weizmann Institute of Science Rehovot, Israel

Received April 1, 1980. The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes.


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