Nucleic Acids Research, 1980, Vol. 8, No. 10 2193-2212
© 1980
Articles |
Sequence analysis and in vitro maturation of five precursor 5S RNAs from Bacillus Q
Biochemisch Lsboratorium, Vrije Universiteit De Boelelaan 1085, 1081 HV Amsterdam, Netherlands
Received March 17, 1980. Bacillus Q, which is closely related to B.subtilis, contains at least six different precursors of 5S rRNA. The complete nucleotide sequences of four of these precursors, as well as the major part of the sequence of a fifth one, have been determined. They all contain the same 5'-terminal non-conserved segment which is to a large degree homologous with the corresponding segment of the B.subtilis p5S RNAs (Sogin, M.L., Pace, N.R., Rosenberg, M., Weissman, S.M. (1976) J. Biol. Chem. 251, 3480–3488) . On the other hand the 3'-terminal non-conserved sequences of the various Bacillus Q precursors show considerable differences both in length and in nucleotide sequence, while there is also little or no homology with the 3'-terminal non-conserved sequence of the B.subtilis precursors. Bacillus Q p5S RNAs do not possess tetranucleotide repeats around the sites which are cleaved during maturation, as does B.subtilis p5S RNA. Like in B.subtilis however, the cleavage sites are contained within a double-helical region of the precursor solecules. Crude ENAse M5 isolated from various Bacillus strains can maturate the Bacillus Q p5S RNAs with high efficiency. Despite considerable differences in primary structure between the precursors from the various strains, each ikse M5 preparation can maturate all these precursors with about the same efficiency.