Nucleic Acids Research, 1980, Vol. 8, No. 10 2275-2294
© 1980
Articles |
Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence
Department of Genetics and Development, University of Illinois Urbana, IL 61801, USA Thimann Laboratories, University of California at Santa Cruz Santa Cruz, CA 95064, USA
Received May 2, 1980. We have derived a secondary structure model for 16S ribosomal RNA on the basis of comparative sequence analysis, chemical modification studies and nuclease susceptibility data. Nucleotide sequences of the E. coli and B. brevis 16S rRNA chains, and of RNAse T1 oligomer catalogs from 16S rRNAs of over 100 species of eubacteria were used for phylogenetic comparison. Chemical modification of G by glyoxal, A by m-chloroperbenzoic acid and C by bisulfite In naked 16S rRNA, and G by kethoxal in active and inactive 30S ribosomal subunits was taken as an indication of single stranded structure. Further support for the structure was obtained from susceptibility to RNases A and T1. These three approaches are in excellent agreement. The structure contains fifty helical elements organized into four major domains, in which 46 percent of the nucleotides of 16S rRNA are involved in base pairing. Phylogenetic comparison shows that highly conserved sequences are found principally in unpaired regions of the molecule. No knots are created by the structure.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Liu, C. Lozupone, M. Hamady, F. D. Bushman, and R. Knight Short pyrosequencing reads suffice for accurate microbial community analysis Nucleic Acids Res., September 25, 2007; 35(18): e120 - e120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Smit, J. Widmann, and R. Knight Evolutionary rates vary among rRNA structural elements Nucleic Acids Res., May 11, 2007; 35(10): 3339 - 3354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kirthi, B. Roy-Chaudhuri, T. Kelley, and G. M. Culver A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity RNA, December 1, 2006; 12(12): 2080 - 2091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zemann, A. op de Bekke, M. Kiefmann, J. Brosius, and J. Schmitz Evolution of small nucleolar RNAs in nematodes. Nucleic Acids Res., January 1, 2006; 34(9): 2676 - 2685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yassin, K. Fredrick, and A. S. Mankin Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics PNAS, November 15, 2005; 102(46): 16620 - 16625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pichon and B. Felden From The Cover: Small RNA genes expressed from Staphylococcus aureusgenomic and pathogenicity islands with specific expression among pathogenic strains PNAS, October 4, 2005; 102(40): 14249 - 14254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Noller RNA Structure: Reading the Ribosome Science, September 2, 2005; 309(5740): 1508 - 1514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Miranda-Rios, M. Navarro, and M. Soberon A conserved RNA structure (thi box) is involved in regulation of thiamin biosynthetic gene expression in bacteria PNAS, July 19, 2001; (2001) 161168098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Gupta Protein Phylogenies and Signature Sequences: A Reappraisal of Evolutionary Relationships among Archaebacteria, Eubacteria, and Eukaryotes Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1435 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Stern, T Powers, L. Changchien, and H. Noller RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA Science, May 19, 1989; 244(4906): 783 - 790. [Abstract] [PDF]
|
|
|
|
|
|
R. GUPTA, J. M. LANTER, and C. R. WOESE Sequence of the 16S Ribosomal RNA from Halobacterium volcanii, an Archaebacterium Science, August 12, 1983; 221(4611): 656 - 659. [Abstract] [PDF]
|
|
|
|
|
|
H. Noller and C. Woese Secondary structure of 16S ribosomal RNA Science, April 24, 1981; 212(4493): 403 - 411. [Abstract] [PDF]
|
|
|
|
|
|
J. Miranda-Rios, M. Navarro, and M. Soberon From the Cover: A conserved RNA structure (thi box) is involved in regulation of thiamin biosynthetic gene expression in bacteria PNAS, August 14, 2001; 98(17): 9736 - 9741. [Abstract] [Full Text] [PDF]
|
|