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Nucleic Acids Research, 1980, Vol. 8, No. 10 2295-2300
© 1980

Articles

DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription

Francis H. Martin and Ignacio Tinoco, Jr.

Department of Chemistry and Chemical Biodynamics Laboratory, University of California Berkeley, CA 94720, USA

Received March 5, 1980. A DNA-RNA hybrid oligonucleotide duplex, dC(pA) 5pG:rC(pU)5pG, which contains a (dA:rU)5 sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT)5 sequence, rC(pA)5pG:dC(pT)5pG. This result provides an explanation for the finding that most primary RNA transcripts terminate in several consecutive rU residues, but not rA residues. It strongly supports the idea that instability of the DNA-RNA hybrid at the growing point of transcription plays a role in termination of transcription.


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