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Nucleic Acids Research, 1980, Vol. 8, No. 11 2453-2460
© 1980

Articles

Selective blotting of restriction DNA fragments on nitrocellulose membranes at low salt concentrations

Yoshikuni Nagamine, André Sentenac and Pierre Fromageot

Service Biochimie, Département de Biologie, Centre d'Etudes Nucléaires de Saclay B.P. No. 2, 91190, Gif-sur-Yvette, France

Received April 3, 1980. The pattern of restriction DNA fragments transferred from agarose gel to nitrocellulose membranes (Southern's technique) can be affected by the salt concentration of the transfer solvent.


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