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Nucleic Acids Research, 1980, Vol. 8, No. 11 2517-2526
© 1980

Articles

Melting fine structure of filamentous fungus nuclear DNA

G. Szécsi and A. Dobrovolszky

Research Institute for Plant Protection H-1525 Budapest, P.O. Box 102, Hungary

Received April 2, 1980. Melting fine structure of the nuclear DNA isolated from the filamentous fungus Fusarium graminearum Schwabe is presented. Optical melting profiles of nuclear DNA were analyzed by using a combination of curve fitting and derivative techniques. The "melting components" were obtained from the derivative curve by a simple decomposition technique. Differential optical melting curves of unsheared nuclear DNA indicate the presence of 15 "melting components" in filamentous fungus nuclear genome. It should be emphasized that the "melting components" observed here are different from the "thermalites" which can be observed in bacteriophage DNA. The "melting components" reported here represent the separately melting of large "blocks" of fungus nuclear DNA.


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