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Nucleic Acids Research, 1980, Vol. 8, No. 11 2527-2536
© 1980


Articles

A new conformation-specific infrared band of A-DNA in films

Walter Pohle and Hartmu Fritzsche

Academy of Sciences of the G.D.R., Central Institute of Microbiology and Experimental Therapy, Department of Biophysical Chemistry DDR-69 Jena, Beutenbergstr. 11, GDR

Received April 1, 1980. A band at 1185 cm–1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm–1 band has been assigned tentatively to a vibration of the sugar-phosphate backbone with a fairly high contribution from the sugar moiety.

Drastic dehydration of the DBA films is accompanied by a continuous intensity decrease of the 1185 cm–1 band indicating a collapse of the A form.


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