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Nucleic Acids Research, 1980, Vol. 8, No. 11 2537-2546
© 1980

Articles

A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Christopher R. Erwin*, Richard A. Maurer= and John E. Donelson

*Department of Biochemistry, University of Iowa Iowa City, IA 52242, USA =Physiology and Biophysics, University of Iowa Iowa City, IA 52242, USA

Received March 13, 1980. Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.


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