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Nucleic Acids Research, 1980, Vol. 8, No. 12 2725-2736
© 1980

Articles

Analysis of recombination in mammalian cells using SV4O genome segments having homologous overlapping termini

Peter Upcroft*, Bernard Carter and Chev Kidson

Queensland Institute of Medical Research Brisbane, Australia 4006

* To whom correspondence should be addressed

Received April 1, 1980. Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has been devised for analysing host cell recombination functions.


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C. Miller and H. Temin
High-efficiency ligation and recombination of DNA fragments by vertebrate cells
Science, May 6, 1983; 220(4597): 606 - 609.
[Abstract] [PDF]


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