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Nucleic Acids Research, 1980, Vol. 8, No. 13 2921-2938
© 1980

MOLECULAR BIOLOGY

Transfer RNA genes of Drosophila melanogaster

Robert Dudler*, Albert H. Egg*, Eric Kubli*, Spiros Artavanis-Tsakonas+, Walter J. Gehring+, Ruth Steward+ and Paul Schedl{dagger}

*Zoologisches Institut der Universität Zürich-Irchel Winterthurerstrasse 190, CH-8057 Zürich, Switzerland +Department of Cell Biology, Princeton University Biozentrum, Basel, Switzerland {dagger}Department of Biology, Princeton University Princeton, NJ 08544, USA

Received May 7, 1980. Three recombinant plasmids containing randomly sheared genomic D.melanogaster tRNAs have been identified and characterized in detail. One of these, the plasmid 14C4, has a D.melanogaster (Dm) DNA segment of 18 kb, and has three tRNA2Arg and two tRNAASN genes. The second plasmid, 38B10, has tRNAHis genes, while the third plasmid, 63H5, contains coding sequences for tRNA2Arg The Dm DNA segments in each recombinant plasmid are derived from unique cytogenetic loci. 14C4 is from 84 F, 38B10 is from 48 F and 63H5 is from 70 A.


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