Nucleic Acids Research, 1980, Vol. 8, No. 14 3105-3124
© 1980
MOLECULAR BIOLOGY |
Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8
Department of Molecular Biology, University of Geneva 30, quai Ernest-Ansermet, 1211 Geneva 4, Switzerland
+To whom reprints requests should be addressed
Received May 21, 1980.
We present the sequences at the 5' and 3' ends of one hsp 70 gene variant which is derived from the chromosomal locus 87A7. The 5' end of the hsp 70 mRNA has also been determined. 550 bp upstream from the 5' end of the hsp 70 mRNA, there is a very A+T rich region shown by heteroduplex analysis to be also present at the same position in other hsp 70 genes9. The 5' end of the hsp 70 mRNA was found 26 bp after a characteristic "Hogness box". The first ATG codon was found 250 bp downstream from the 5' end of the hsp 70 mRNA. We also determined the termination codon at the 3' end of the hsp 70 gene. Comparisons with other genes are discussed.
*Present address: Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, P.O.B. 521, Hungary.
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