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Nucleic Acids Research, 1980, Vol. 8, No. 14 3157-3174
© 1980

MOLECULAR BIOLOGY

Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu ‘lig’ gene

P. Ghelardini*, L. Paolozzi+ and J.C. Liebart{dagger}

*Laboratorio di Biologia Cellulare via Romagnosi 18A, Roma, Italy +Centro Acidi Nucleici del C.N.R. via Romagnosi 18A, Roma, Italy {dagger}Unitè de Physiologie de la Replication, Institut Pasteur 75724, Paris Cedex 15, France

Received May 12, 1980. Restoration of ligase activity has been observed in E. coli K12 ligts7 strain lysogenic for Mu , in presence as well in absence of lysogenic imunity. This restoration consist in phenotypic reversal of temperature sensitivity of E. coli ligts7 which also regain the ability to sustain the complete growth cycle of T4 lig– phages.

It is possible to put under the control of the gal operon the expression of the viral gene responsible for the restoration effect.

This new gene of Mu has been named ‘lig’.

A 5 kb fragment responsible for the reported effects and localized between genes gam and lys of Mu genome has been cloned in pBR322. This recombinant plasnid used for transforming ligts7 strain restores in it normal behaviour for ligation of Okazaki pieces.


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