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Nucleic Acids Research, 1980, Vol. 8, No. 14 3193-3214
© 1980

MOLECULAR BIOLOGY

Comparison of polysomal and nuclear poly(A}-containing RNA populations from normal rat liver and Novikoff hepatoma

Yassemi G. Capetanaki and Angel Alonso

Institute of Experimental Pathology, German Cancer Research Center Heidelberg, GFR

Received May 7, 1980. Polysomal and nuclear poly(A)-containing RNA of normal rat liver and Novikoff hepatoma cells have been compared by cDNA.RNA hybridization kinetics. Homologous hybridization reactions revealed a total kinetic complexity of about 1.6x1O10 and 1.38x 1O10 daltons for liver and Novikoff mRNA respectively. The high abundance component present in liver cannot be detected in Novikoff. It was found from heterologous reactions that about 3O% by weight of mRNA sequences are specific to liver. Determination of the nuclear poly(A)-containing RNA complexities revealed that about 5.5% and 4% of the haploid genome is expressed in the liver and Novikoff respectively. In a heterologous reaction, up to 30% of the liver cDNA failed to form hybrids with Novikoff nuclear RNA. Cross hybridizations have further revealed abundance shifts in both nuclear and polysomal RNA populations. Some sequences abundant in liver are less abundant in Novikoff and some rare liver sequences are relatively abundant in Novikoff.


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