Nucleic Acids Research, 1980, Vol. 8, No. 15 3355-3369
© 1980
MOLECULAR BIOLOGY |
Studies of RNA release reaction catalyzed by E. coli transcription termination factor rho using isolated ternary transcription complexes+
Department of Biochemistry, Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, NY 10461, USA
To whom all the correspondence should be addressed. Present Address: Department of Pharmacological Sciences, State University of New York, Stony Brook, N.Y.11794
Received April 18, 1980.
Protein factor rho catalyzes site-specific termination of transcription in a reaction requiring hydrolysis of nucleoside triphosphate with eventual release of RNA from RNA polymerase and DNA template. We have characterized the rho-catalyzed RNA release reaction using isolated transcription complexes. Transcription complexes containing T7 D111 DNA, RNA polymerase, and 3H-labeled nascent RNA were formed and isolated by gel filtration on an Agarose 5M column. When the ternary complexes were incubated with rho factor in the presence of ATP or dATP, significant amounts of nascent RNA were released from the complexes as determined in a membrane filtration assay. Gel electrophoretic analysis of RNA has revealed that rho releases selected species of discrete-sized RNA from among those originally present in the ternary complexes. These results show that rho essentially acts to release RNA from those ternary complexes which have come to pause, and that this reaction proceeds in a discrete step separately from the pausing of RNA synthesis. Under the conditions used, the extent of RNA release widely varied at individual pausing sites and thus the action of rho exhibited certain site-selectivity.
+This work was supported in part by research grants from the National Institutes of Health (GM 28069) and the American Cancer Society (NP 309F).
*0n leave from Department of Biochemistry, Institute for Virus Research, Kyoto University, Kyoto, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. V. Sukhodolets, S. Garges, and S. Adhya Ribosomal protein S1 promotes transcriptional cycling RNA, August 1, 2006; 12(8): 1505 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hinde, P. Deighan, and C. J. Dorman Characterization of the Detachable Rho-Dependent Transcription Terminator of the fimE Gene in Escherichia coli K-12 J. Bacteriol., December 15, 2005; 187(24): 8256 - 8266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-E. Kim, K. Shigesada, and S. S. Patel Transcription Termination Factor Rho Contains Three Noncatalytic Nucleotide Binding Sites J. Biol. Chem., April 23, 1999; 274(17): 11623 - 11628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Burns, W. L. Nowatzke, and J. P. Richardson Activation of Rho-dependent Transcription Termination by NusG. DEPENDENCE ON TERMINATOR LOCATION AND ACCELERATION OF RNA RELEASE J. Biol. Chem., February 19, 1999; 274(8): 5245 - 5251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Richardson and J. P. Richardson Rho-dependent Termination of Transcription Is Governed Primarily by the Upstream Rho Utilization (rut) Sequences of a Terminator J. Biol. Chem., August 30, 1996; 271(35): 21597 - 21603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Li, S W Mason, and J Greenblatt Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription. Genes & Dev., January 1, 1993; 7(1): 161 - 172. [Abstract] [PDF]
|
|