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Nucleic Acids Research, 1980, Vol. 8, No. 15 3467-3480
© 1980

MOLECULAR BIOLOGY

Alterations in tRNA isoaccepting species during erythroid differentiation of the Friend leukemia cell

Victor K. Lin and Paul F. Agris

Division of Biological Sciences, University of Missouri Columbia, MO 65211, USA

Received January 17, 1980. The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAPhe, tRNAile, and tRNAval species were maintained by the cells during differentiation; whereas the relative amounts of some of the isoacceptor tRNAs for the other 13 amino acids changed significantly. Fluctuations in amounts of isoacceptors occurred between 36 and 72 hr after addition of dimethylsulfoxide, corresponding to globin mRNA appearance and hemoglobin synthesis, respectively. In most cases, the predominant tRNA isoacceptors of uninduced cells were retained throughout differentiation. Notable exceptions were tRNA species for threonine, proline, and methionine. Some of the isoacceptors occurring in relatively smaller amounts were not expressed at all times. These changes possibly reflect the cell's functional adaptation of tRNA in differentiation for hemoglobin synthesis.


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P. Agris and I. Campbell
Proton nuclear magnetic resonance of intact Friend leukemia cells: phosphorylcholine increase during differentiation
Science, June 18, 1982; 216(4552): 1325 - 1327.
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