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Nucleic Acids Research, 1980, Vol. 8, No. 16 3505-3516
© 1980

MOLECULAR BIOLOGY

Mapping of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA

James E. Jurgenson* and Don P. Bourque

*Committee on Genetics and Nutrition and Food Science, University of Arizona Tucson, AZ 85721, USA Departments of Biochemistry Nutrition and Food Science, University of Arizona Tucson, AZ 85721, USA

Received July 16, 1980. Nicotiana tabacum chloroplast DNA contains two copies each of 16S and 23S rRNA genes. These genes are located in an inverted order as determined from restriction fragment mapping and Southern hybridization to restriction fragments. The position of these genes on the N. tabacum chloroplast DNA molecule has been established relative to a complete map of SalI and SmaI restriction enzyme cleavage sites.


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