Skip Navigation

This Article
Right arrow Print PDF (481K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sneider, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sneider, T. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 17 3829-3840
© 1980

MOLECULAR BIOLOGY

The 5'-cytosine in CCGG1 is methylated in two eukaryotic DNAS and Msp I is sensitive to methylatlon at this site

Thomas W. Sneider

Department of Biochemistry and Graduate Faculty of Cellular and Molecular Biology, Colorado State University Fort Collins, CO 80523, USA

Received May 21, 1980. Novikoff rat hepatoma and bovine liver DNAs were digested with Msp I or Hpa II. Restriction fragments were end-labeled using [{alpha}-32P]-dCTP and the Klenow fragment of E.coli DNA polymerase I and then digested to 2'-deoxyribonucleo-side-3' -monophosphates using micrococcal nuclease and spleen phosphodiesterase. Mononucleotides were separated by two-dimensional thin layer chromatography, localized by radioautography, and the [32P]-label quantitated by scintillation spectrometry. This method, based on known specificities of Msp I and Hpa II, shows that CCGG, CMGG, and MCGG (M refers to 5-methylcytosine) occur at frequencies of 89.6%, 1.4%. and 9.0%, respectively, in the rat DNA and at 41.6%, 48.3%, and 10.0%, respectively, in the bovine DNA. [32P] recovery in 3'-5-MedCMP from end-labeled Msp I digests was negligible compared to recovery from Hpa II digests. Hence, Msp I is sensitive to methylation at the 5' cytosine in the sequence CCGG.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
R. Muschel, G Khoury, P Lebowitz, R Koller, and R Dhar
The human c-ras1H oncogene: a mutation in normal and neoplastic tissue from the same patient
Science, February 18, 1983; 219(4586): 853 - 856.
[Abstract] [PDF]

Home page
 ScienceHome page
M Ehrlich and R. Wang
5-Methylcytosine in eukaryotic DNA
Science, June 19, 1981; 212(4501): 1350 - 1357.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.