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Nucleic Acids Research, 1980, Vol. 8, No. 17 3865-3874
© 1980

ENZYMOLOGY

DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage

Akio Sugino{dagger},+, N.Patrick Higgins{dagger},* and Nicholas R. Cozzarelli{dagger}

{dagger}Department of Biochemistry, University of Chicago Chicago, IL 60637 +Laboratory of Molecular Genetics, National institute of Environmental Health Sciences Research Triangle Park, NC 27709 *Division of Biochemistry, University of Wyoming Laramie, WY 82071, USA

Received May 16, 1980. Escherichia coli DNA gyrase contains a 1:1 ratio of protomers coded by the genes gyrA and gyrB. This along with previous results shows that the enzyme has two copies of each protomer and thus a molecular weight of 400,000. Abortion of the gyrase reaction results in double-strand breakage of the DNA and covalent attachment of both gyrA protomers to the 5'-cut ends. We conclude that the gyrA protomer contains a critical part of the active site for the concerted breakage and reunion reaction of gyrase, the topoisomerase activity of the enzyme.


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