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Nucleic Acids Research, 1980, Vol. 8, No. 17 3947-3964
© 1980

MOLECULAR BIOLOGY

E. coli RNA polymerase-rRNA promoter interaction and the effect of ppGpp

Jantien Hamming, Geert AB and Max Gruber

Biochemisch Laboratorium, The University Nijenborgh 16, 9747 AG Groningen, The Netherlands

Received June 27, 1980. The interaction between RNA polymerase and the E. coli r(ibosomal) RNA promoters of the rrnX arid rrnE operon was studied with the filter-binding technique. Quantitative differences were observed between the rrnX and rrnE promoters: stable rrnX promoter complexes are formed faster, and are less sensitive towards heparin and salt than stable rrnE promoter complexes. The effect of ppGpp, the specific inhibitor of rRNA synthesis, on rrn promoter complex formation was studied. In the presence of ppGpp complexes are formed which cannot be trapped in a transcription complex by addition of the start nucleotides, and are therefore considered to be non-productive. A tentative model for the action of ppGpp is proposed.


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