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Nucleic Acids Research, 1980, Vol. 8, No. 17 3965-3974
© 1980

CHEMISTRY

Salt-induced conformational changes of poly(dA-dT)

Michaela Vorlicková, Jaroslav Kypr, Vladimir Kleinwächter and Emil Palecek

Institute of Biophysics, Czechoslovak Academy of Sciences 612 65 Brno, Královopolská 135, Czechoslovakia

Received June 9, 1980. Conforsational changes of poly(dA-dT).poly(dA-dT) induced by increasing ionic strength ware studied using CD spectrascopy. It was found that a pronounced noncooperative inversion of the long-wavelength part of the CD spectrum of poly(dA-dT).poly(dA-dT) occurred at high concentretions of CeF in solution. It was suggested that a great difference between the geoaetries of the purine and pyrisidin residues in the helix was characteristic of the structure of poly(dA-dT).poly(dA-dT) in concentrated CsF solutions.


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