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Nucleic Acids Research, 1980, Vol. 8, No. 18 4057-4074
© 1980

MOLECULAR BIOLOGY

Synthesis of human fibroblast interferon by E. coli

David V. Goeddel*, H.Michael Shepard*, Yelverton Elizabeth*, David Leung*, Roberto Crea**, Alan Sloma and Sidney Pestka

Roche Institute of Molecular Biology Nutley, NJ 07110, USA *Departments of Molecular Biology Genentech Inc., 460 Pt. San Bruno Blvd, South San Francisco, CA 94080 **Departments of Organic Chemistry Genentech Inc., 460 Pt. San Bruno Blvd, South San Francisco. CA 94080

Received August 12, 1980. A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 107 units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.


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