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Nucleic Acids Research, 1980, Vol. 8, No. 18 4283-4304
© 1980

CHEMISTRY

Thermal stability of poly (A) and poly (U) complexes in H2O and D2O: isotopic effects on critical temperatures and transition widths

A. Cupane*, E. Vitrano*, P.L.San Biago, F. Madonia and M.U. Palma*

Istituto di Fisica dell' Università Via Archirafi, 36, 90123 - Palermo, Italy *Gruppo Nazionale Struttura della Materia of the Consiglio Nazionale delle Richerche Via Archirafi, 36, 90123-Palermo, Italy

Received May 30, 1980. This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A)·n poly(U) (n =1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered structures, and it was extended to a wide range of salt concentations at neutral pH.

The dependence of stability of polymer helical order on hydrogen mass does not appear to be merely attributable to the stronger intrahelical deuterium bonding. Substituting Deuterium for Hydrogen implies a probably predominant modulation of the entropy term of polymer-solvent interaction.

Effects of deuteration on the width of the 2{poly(A)·poly (U)} -> poly(A)·2poly(U)+poly(A) interconversion reaction were also observed. They bear on the role of polymer-solvent interaction on pattern recognition leading to formation of ordered structures. They also bear on the role of the same interaction on the "breathing" of ordered structures of this type.


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