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Nucleic Acids Research, 1980, Vol. 8, No. 2 253-264
© 1980

Article

Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA

Gösta Winberg and Marie-Louise Hammarskjöld

Department of Virology, Karolinska Institutet, c/o SBL, S 105 21 Stockholm, Sweden

Received December 1, 1979. A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of the individual fragments from the paper with 1 M NaCl. DNA isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with E coli DNA-polyraerase I, T4 DNA-polymerase and T4 poly-nucleotide kinase.

We have used the method to construct restriction endonuclease maps of adenovirus type 16 DNA.


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