Skip Navigation

This Article
Right arrow Print PDF (4705K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Stellwag, E. J.
Right arrow Articles by Dahlberg, A. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stellwag, E. J.
Right arrow Articles by Dahlberg, A. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 2 299-317
© 1980

Article

Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper

Edmund J. Stellwag and Albert E. Dahlberg*

Department of Physiological Chemistry, University of Wisconsin, Madison, Wl 53706, USA

Received October 11, 1979.

A method has been developed for the electrophoretic transfer of DNA,RNA,protein and ribonucleoprotein particles from a variety of gels onto diarobenryloxymethyl (DBM) paper. Conditions for the electrophoretic transfer of these macromolecules have been optimized to allow for nearly quantitative transfer and covalent coupling. DNA and RNA electrophoretically transferred to DBM-paper retain their ability to hybridize with specific probes. The high efficiency of transfer and the high capacity of DBM-paper for nucleic acids makes possible the sensitive detection of specific nucleotide sequences. Similar efficiency is achieved in electrophoretic transfer and covalent coupling of proteins to DBM-paper. Macromolecules can also be electrophoretically transferred and bound to DBM-paper incapable of covalent bond formation. Their elution from the paper in high salt provides a new and useful preparative method for isolation of DNA,RNA and protein.

*Permanent address: Division of Biology and Medicine, Brown University, Providence, Rhode Island, 02912, USA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
P. L. Dolan, Y. Wu, L. K. Ista, R. L. Metzenberg, M. A. Nelson, and G. P. Lopez
Robust and efficient synthetic method for forming DNA microarrays
Nucleic Acids Res., November 1, 2001; 29(21): e107 - e107.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
F Marashi, L Baumbach, R Rickles, F Sierra, J. Stein, and G. Stein
Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner
Science, February 5, 1982; 215(4533): 683 - 685.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.