Nucleic Acids Research, 1980, Vol. 8, No. 2 337-350
© 1980
Article |
Assignment of reovirus mRNA ribosome binding sites to virion genome segments by nucleotide sequence analyses
Roche Institute of Molecular Biology, Nutley, NJ 07110, USA
Received August 21, 1979.
All ten reovirus genome RNA segments were radiolabeled at their 3'-termini by incubation with RNA ligase and 32pCp. The extent of radiolabeling was similar for each of the double-stranded RNAs in the genome segment mixture. Radioactivity was equally distributed between the separated plus and minus strands indicating that the 5'-cap in plus strands did not block 3'-end-labeling of minus strands. The 3'-termini of the four S and three M segments included the common sequences: ...U-A-G-C in minus strands and ...U-C-A-U-C in plus strands. By comparing the minus strand 3'-sequences with 5'— sequences of reovirus mRNAs, small-size genome segments S2, S3 and S4 were correlated with the previously sequenced initiation fragments s46, s45 and s54 derived from small class mRNAs. Medium-size genome segments Ml, M2 and M3 similarly were correlated with fragments m30, m52 and m44, respectively. The N-termlnal amino acid sequences deduced from the mRNA nucleotide sequences can now be assigned to the nascent chains of particular reovirus proteins.
*Permanent address: University of Warsaw, Institute, Institute of Experimental Physics, Department of Biophysics, Warsaw, poland.