Nucleic Acids Research, 1980, Vol. 8, No. 20 4613-4620
© 1980
CHEMISTRY |
Pyrimidine-specific chemical reactions useful for DNA sequencing
Department of Chemistry, University of California Davis, CA 95616, USA
Received August 20, 1980. Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
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