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Nucleic Acids Research, 1980, Vol. 8, No. 21 4841-4850
© 1980

MOLECULAR BIOLOGY

Sequence of 863 nucleotides encompassing the PstI site of the yeast 2 µm plasmid

T. C. Elleman and J. Hindley

Department of Biochemistry, University of Bristol, Medical School, University Walk Bristol, BS8 1TD, UK

Received September 26, 1980. The sequence of part of the larger unique region of the yeast 2µm plasmid cloned in pMB9 has been determined. The sequence extends from the single EcoRI site in this region to the AvaI site and includes the single PstI site and HpaI site. A notable feature of this sequence is the presence of tandem repeats of 124 residues beginning at the HpaI site and extending beyond the AvaI site. The sequence was determined independently by both the Maxam-Gilbert procedure applied to isolated restriction fragments, and by the chain-termination procedure applied to restriction fragments cloned in the single-stranded phage M13mp2 and purified by plaque selection.


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