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Nucleic Acids Research, 1980, Vol. 8, No. 21 4919-4926
© 1980

MOLECULAR BIOLOGY

The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transription

A.A. Bayev, O.I. Georgiev*, A.A. Hadjiolov*,, M.B. Kermekchiev*, N. Nikolaev*, K.G. Skryabin and V.M. Zakharyev

Institute of Molecular Biology, Academy of Sciences of the USSR Vavilov str. 32, Moscow B-312, USSR *Institute of Molecular Biology, Bulgarian Academy of Sciences 1113 Sofia, Bulgaria

Correspondence should be addressed to: Prof. Dr. A.A.Hadjiolov, Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Received August 12, 1980. The 5'-terminal coding sequence for the 37 S precursor to rRNA of Saccharomyces cerevisiae is identified by reverse trsnscriptase extension and protection mapping with nuclease S1. The sequence of a 419 bp rDNA fragment containing the transcription initiation site and its adjacent region is determined.


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