Skip Navigation

This Article
Right arrow Print PDF (1845K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bonne, C.
Right arrow Articles by de Recondo, A.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bonne, C.
Right arrow Articles by de Recondo, A.-M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 21 4955-4968
© 1980

MOLECULAR BIOLOGY

Single-strand DNA binding protein from rat liver: interactions with supercoiled DNA

Catherine Bonne, Michel Duguet and Anne-Marie de Recondo

Unité d'Enzymologie, Institut de Recherches Scientifiques sur le Cancer BP no.8, 94800 Villejuif, France

Received August 5, 1980. As shown by competition experiments, the single-strand DNA binding protein from normal rat liver (S25) interacts preferentially with supercoiled DNA compared to relaxed DNA duplexes. When followed both by sedimentation analysis and by nitrocellulose filter assay, the binding of S25 to SV40 supercoiled DNA (FI) appears to be non-cooperative. Saturation is reached at a protein to DNA weight ratio of about 2. The S25-DNA complexes prefixed with glutaraldehyde appear as beaded structures having an average of 14 to 16 beads per SV40 DNA molecules. Cross-linking of S25 bound to SV40 DNA by dimethyl suberimidate allows to detect oligomeric structures containing a maximum of twenty monomers of S25. When complexes are treated by glutaraldehyde, 10 % of the genome become resistant against micrococcal nuclease. Moreover, S25 affects the DNA helical structure. Superhelical forms are generated by the association of S25 with SV40 DNA, in the presence of nicking-closing enzyme.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.