Skip Navigation

This Article
Right arrow Print PDF (2376K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ordahl, C. P.
Right arrow Articles by Largen, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ordahl, C. P.
Right arrow Articles by Largen, M. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 21 4989-5005
© 1980

MOLECULAR BIOLOGY

Structure and developmental expression of the chick {alpha}-actin gene

Charles P. Ordahl*, Shirley M. Tilghman**, Catherine Ovitt*, James Fornwald* and Michael T. Largen{dagger}

*Department of Anatomy, Temple University Medical School Philadelphia, PA 19140, USA **Institute for Cancer Research Fox Chase, Philadelphia, PA 19111, USA {dagger}Department of Microbiology, Temple University Medical School Philadelphia, PA 19140, USA

Received July 25, 1980. Recombinant DNA clones containing chick {alpha}-actin mRNA sequence have been isolated and used as probes to analyze the structure and developmental expression of the chick {alpha}-actin gene. The full length, 2000 nucleotide {alpha}-actin mRNA is detected in poly(A) RNA at early and late stages of in vivo leg muscle development. As expected, the {alpha}-actin mRNA is present at very low levels at early myogenic stages but is a high abundance species in terminally differentiated muscle. However, most of the {alpha}-actin mRNA from fused leg muscle is shorter than 2000 nucleotides, and occurs in relatively discrete size classes. An {alpha}-actin-like mRNA can be detected in poly(A) RHA from early embryonic brain, indicating that transcription of the {alpha}-actin gene may not be strictly muscle-specific at all stages of development.

We have identified at least 3, very short (< 100 base pairs) intervening sequences in the {alpha}-actin gene which was isolated from a chick genomic library. The structure of the chick {alpha}-actin gene differs, therefore, from the structures of actin genes from yeast and Drosophila, both of which contain a single, relatively long, intervening sequence.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
B. Paterson and J. Eldridge
alpha-Cardiac actin is the major sarcomeric isoform expressed in embryonic avian skeletal muscle
Science, June 29, 1984; 224(4656): 1436 - 1438.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.