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Nucleic Acids Research, 1980, Vol. 8, No. 21 5057-5070
© 1980

MOLECULAR BIOLOGY

Ribonucleotide sequences non-adjacent to poly(A) participate in the poly(A)-protein complex in 15S duck globin mRNP particles

Samuel Goldenberg, Alain Vincent and Klaus Scherrer*

Laboratoire de Biochimie de la Différenciation, Institut de Recherche en Biologie Moleculaire, Universite Paris VII - Tour 43, 2 Place Jussieu, 75221 Paris Cedex 05, France

*To whom correspondence should be addressed

Received June 30, 1980. The study of the interaction between mRNA and proteins in the polyribosomal 15 S duck globin messenger ribonucleoprotein complex showed that proteins protect specific mRNA sequences against digestion by the nonspecific micrococcal nuclease (Nucleic Acids Research 6 (8) 2787, 1979). Here we report the isolation of the poly(A)-protein RNP complex from nuclease digested 15 S mRNP by two different methods : sucrose gradient sedimentation and oligo(dT)-cellulose chromatography. We show by fingerprint analysis, that apart from the periodically fragmented poly(A) segment, mRNA sequences adjacent and non-adjacent to the poly(A) segment are protected by the poly(A) binding proteins against nuclease digestion. The duck globin poly (A)-protein RNP complex, with a sedimentation coefficient between 7 S and 10 S, shows a characteristic protein composition, with a major 73,000 MW polypeptide and some minor components. The results are discussed in view of a dynamic ribonucleoprotein structure.


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