Nucleic Acids Research, 1980, Vol. 8, No. 22 5207-5212
© 1980
MOLECULAR BIOLOGY |
The nucleotide sequence of a small (3S) seryl-tRNA (anticodon GCU) from beef heart mitochondria
MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK
Received September 22, 1980.
The primary structure of a 3S serine tRNA from beef heart mitochondria has been determined using our new two-dimensional "read-off" sequencing method (Tanaka, Y., Dyer, T.A. and Brownlee, G.G. (1980) Nucleic Acids Ree. 8, 12591272). When arranged in the "cloverleaf" form it shows unique features since (i) it completely lacks the dihydrouridine arm, (ii) it has an extended "T
" loop, but lacks the T and
residues, and (iii) it has only one minor base, N6(N-threonylcarbamoyl)adenosine, next to the anticodon.
*On leave of absence from Io Istituto Chimica Biologica, 2nd Medical School, University of Naples, Italy
**Present sddress: The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK