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Nucleic Acids Research, 1980, Vol. 8, No. 22 5267-5274
© 1980

MOLECULAR BIOLOGY

DNA sequence of the araC regulatory gene from Escherichia coli B/r

C.Garrett Miyada, Arnold H. Horwitz, Laura G. Cass, Josef Timko and Gary Wilcox

Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles Los Angeles, CA 90024, USA

Received September 8, 1980. The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation In araC, {Delta}(araC0)719, removes all but the last 22 codons of the gene.


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