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Nucleic Acids Research, 1980, Vol. 8, No. 22 5445-5460
© 1980

MOLECULAR BIOLOGY

A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides

Arun K. Seth and Ernest Jay

Department of Chemistry, University of New Brunswick Fredericton, New Brunswick E3B 5A3, Canada

Received July 29, 1980. The efficiencies and problem of sulfonation of several condensing reagents for deoxyoligoribonucleotide synthesis have been studied. While 2,4,6-triisopropylbenzenesulfonyl chloride (TPSCl) gave very low yield and slow rate of coupling, the new 1:3 mixture of TPSCl and tetrazole afforded excellent yield and extremely fast rate of reaction with the lowest rate of sulfonation. The difference and advantages of this mixture over triisopropylbenzenesulfonyl tetrazole (TPSTe) and mesitylenesulfonyl tetrazole (MSTe) are discussed. Mechanisms for the coupling reactions with these condensing reagents to account for the difference in their rates and yields of coupling are discussed.


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