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Nucleic Acids Research, 1980, Vol. 8, No. 22 5491-5506
© 1980

MOLECULAR BIOLOGY

Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method

Ken-ichi Miyoshi, Ting Huang and Keiichi Itakura

Division of Biology, Molecular Genetics Section, City of Hope Research Institute Duarte, CA 91010, USA

Received July 25, 1980. Preparation of the three hexadecanucleotides, dGpTpApTpCpApCpGpApGpGpCpCpCpTpT, dCpGpApCpGpApGpCpGPTpGpApCpApCpC and cTpGpCpCpGpGpCpCpApCpGpApTpGpCpG, is described by a rapid and simple solid-phase method on polyacrylamide supports. The syntheses were performed by the extension of the method described in the previous paper using di and trinucleotides of defined sequences as an incoming 3'-phosphodiester unit. Although the coupling yields to form phosphotriester bonds are slightly lower than those for the homothymidylic acid series, pure polydeoxyribonucleotides of defined sequences can be synthesized without any major difficulty.


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