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Nucleic Acids Research, 1980, Vol. 8, No. 23 5599-5610
© 1980

MOLECULAR BIOLOGY

Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA

Alan R. Kimmel and Richard A. Firtel

Department of Biology, University of California at San Diego La Jolla, CA 92093, USA

Received September 19, 1980. Using S1 nuclease protection experiments and DNA sequencing, we have identified two intervening sequences (introns) within a Dictyostelium gene that codes for a low abundance class mRNA. The two introns are located within the protein coding region of this gene. Both are small ( ~100bp) and extremely (~95%) A + T rich. The splice junction sequences are similar to the splice sites in other eukaryotes. Finally, we have shown that these introns are transcribed as part of a higher molecular weight nuclear precursor.


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[Abstract] [PDF]


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