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Nucleic Acids Research, 1980, Vol. 8, No. 23 5703-5714
© 1980

ENZYMOLOGY

Purification and partial characterization of a DNA polymerase {alpha} species from calf thymus

Frank Grosse and Gerhard Krauss

Abteilung Biophysikalische Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover GFR

Received August 11, 1980. We have purified a DNA polymerase {alpha} species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly(dA)·(dt) 10.


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