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Nucleic Acids Research, 1980, Vol. 8, No. 23 5753-5765
© 1980

CHEMISTRY

Synthesis of the human insulin gene. Part III1. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA

Hansen M. Hsiung, Wing L. Sung, Roland Brousseau, Ray Wu* and Saran A. Narang

Division of Biological Sciences2, National Research Council of Canada Ottawa K1A 0R6, Canada *Section of Biochemistry, Molecular and Cell Biology, Cornell University Ithaca, NY 14853, USA

Received May 2, 1980. A method for phosphorylating a protected deoxyribooligonucleotide containing phosphotriester linkages is described. The modified phosphotriester method of chemical synthesis is further refined in terms of (i) better final deblocking conditions and (ii) new chromatography solvent systems containing acetone-water-ethyl acetate to yield pure oligomers. The effectiveness of these improvements has been demonstrated in the rapid and efficient synthesis of seventeen fragments constituting the sequence of human insulin C-chain DNA.


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