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Nucleic Acids Research, 1980, Vol. 8, No. 24 5983-5991
© 1980

MOLECULAR BIOLOGY

Cloning of human immunoglobulin µ gene and comparison with mouse µ gene

Naoki Takahashi*, Sumiko Nakai and Tasuku Honjo

Department of Genetics, Osaka University Medical School Kita-ku, Osaka 530, Japan

Received November 3, 1980.

We have cloned a 12 kb DNA segment containing human µ gene and its flanking sequence from human fetal liver DNA library using mouse µ gene as a probe. Partial nucleotide sequence determination shows that the cloned DNA contains the sequence encoding human µ chain. This is the first constant region gene of the human heavy chain that is cloned. We have compared human and mouse µ genes by heteroduplex analysis and Southern blot hybridization. The results clearly show that not only the sequence encoding the CH4 domain but also the 5'-flanking (Sµ) sequence is conserved between human and mouse µ genes, suggesting that the nucleotide sequence in the Sµ region has an important biological function, presumably a recognition signal for the class switch recombination as proposed previously.

*On leave from Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan


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