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Nucleic Acids Research, 1980, Vol. 8, No. 24 6007-6017
© 1980

MOLECULAR BIOLOGY

The isolation of cloned cDNA sequen which are differentially expressed in human lymphocytes and fibroblasts

Julian Crampton, Stephen Humphries, Derek Woods and Robert Williamson

Biochemistry Department, St. Mary's Hospital Medical School, University of London London, W2 1PG, UK

Received November 3, 1980. Poly(A)+ RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A)+ RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A)+ RNA but absent or rare in fibroblast poly(A)+ RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.


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