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Nucleic Acids Research, 1980, Vol. 8, No. 24 6043-6058
© 1980

MOLECULAR BIOLOGY

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse

Yoshio Urano, Ryo Kominami, Yukio Mishima and Masami Muramatsu

Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research KamiIkebukuro, Toshima-ku, Tokyo 170, Japan

Received October 21, 1980. Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site was determined by two independent methods: one nuclease S1 protection and the other reverse transcriptase elongation mapping using isolated 45S ribosomal RNA precursor (45S RNA) and appropriate restriction fragments of rDNA. Both methods gave on identical result; 45S RNA had a structure starting from ACTCTTAG---.

Characteristically, mouse rDNA had many T clusters (≥5) upstream the initiation site, the longest being 21 consecutive T's. A pentadeconucleotide, TGCCTCCCGAGTGCA, appeared twice within 260 nucleotides upstream the putative initiation site. No such characteristic sequences were found downstream this site. Little similarity was found in the upstream of the transcription initiation site between the mouse, Xenopus laevis and Saccharomyces cerevisiae rDNA.


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