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Nucleic Acids Research, 1980, Vol. 8, No. 24 6175-6188
© 1980

MOLECULAR BIOLOGY

A cDNA clone from Zea mays endosperm sucrose synthetase mRNA

Martin Geiser, Hans-Peter Döring, Johannes Wöstemeyer1, Ute Behrens, Edith Tillmann and Peter Starlinger

Institut für Genetik, Universität Köln Weyertal 121, 5000 Köln 41, GFR

Received July 21, 1980.

A cDNA clone for maize endosperm sucrose synthetase of 620 nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoted translation.

In Southern blotting to genomic BamHI-digested DNA, a single band only of ~20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.

1present address: Dr.Johannes Wöstemeyer, Institut für Organische und Biochemie der Universität Hamburg, Martin-LutherKing-Platz 6, 2000 Hamburg 3, FRG.


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