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Nucleic Acids Research, 1980, Vol. 8, No. 3 425-440
© 1980

Articles

Sequences hybridizing to mRNA, oligo(dT) and dsRNA from pre-mRNA are contiguous in the cloned mouse DNA fragments

O.N. Tokarskaya, N.A. Tchurikov, P.L. Ivanor, D.A. Kramerov and A.P. Ryskov

Institute of Molecular Biology, USSR Academy of Sciences Moscow B-334, Vavilov street 32, USSR

Received January 2, 1980. Fragments from the DNA of mouse embryos produced by restriction endonuclease HindIII were cloned in pBR322 plasmid and examined for the ability to hybridize in situ with [32P] labeled cDNA synthesized from the polysomal poly(A)+ template. Several of the selected clones were examined for the presence of specific sequences inside the cloned mouse DNA fragments by the blotting procedure of Southern /1/. The data obtained indicate that the majority of the cloned mouse DNA fragments contained sequences hybridizing with cDNA, oligo(dT) and double-stranded regions from pre-mRNA. The results of hybridizatian experiments and double digestion with HindIII+HaeIII endonucleases provide evidence that these sequences could be contiguous in the given restriction DNA fragments.


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