Skip Navigation

This Article
Right arrow Print PDF (1957K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zakut, R.
Right arrow Articles by Mory, Y. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zakut, R.
Right arrow Articles by Mory, Y. Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 3 453-466
© 1980

Articles

Structure of immunoglobulin {gamma}2b heavy chain gene cloned from mouse embryo gene library

Rina Zakut, David Givol and Yves Y. Mory

Department of Chemical Immunology, The Weizmann Institute of Science Rehovot, Israel

Received December 21, 1979. A mouse DNA clone containing the constant part of the immunoglobulin {gamma}2b heavy chain was isolated from a mouse gene library. The library was constructed in Charon 4A from a partial EcoRI digest of mouse embryo DNA and was screened with a plasmid (p{gamma}(11)7) containing a cDNA insert of the heavy chain constant region of the plasmacytoma MPC-11 (1). The Charon 4A clone contains a 14 kb insert which is cleaved by EcoRI into a 6.8 kb and 7.2 kb fragments, of which only the 6.8 kb contains the sequence for {gamma}2b heavy chain. Restriction analysis and partial sequence of the insert in py(11)7 enabled us to obtain three fragments corresponding to the 5' (amino acid 161–302) iiiddle(amino acid 302–443) and 3' (mostly non coding 107 bp) regions of the constant region. Restriction analysis of the Charon 4A clone and hybridisation to these nick translated fragments revealed that the {gamma}2b constant region gene contains about 1.5 kb and has three intervening sequences.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.