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Nucleic Acids Research, 1980, Vol. 8, No. 3 567-572
© 1980

Articles

Syntheses of some fluorescent ribonucleotides

K.C. Tsou and K.F. Yip

Harrison Department of Surgical Research and Department of Pharmacology, School of Medicine, University of Pennsylvania Philadelphia, PA 19104, USA

Received January 7, 1980. Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phoephodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.


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