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Nucleic Acids Research, 1980, Vol. 8, No. 5 1009-1022
© 1980

Articles

Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata

G.D. Cark-Walker* and A.A. Azad+

*Department of Genetics, Research School of Biological Sciences, Australian National University P.O. Box 475, Canberra City, 2601, Australia +Molecular Biology Unit, Research School of Biological Sciences, Australian National University P.O. Box 475, Canberra City, 2601, Australia

Received December 7, 1979. We have shown that 2.8 and 3.1 urn circular DNA molecules, previously reported to be present in Saccharomyces cerevisiae and Torulopsis qlabrata respectively, contain sequences hybridizing to cytoplasmic ribosomal RNAs. In S. cerevisiae the 2.8 pm circular DNA appears to be identical to the rDNA repeating unit from nuclear DNA, both in length (approximately 9000 base pairs) and in the location of the 25, 18 and 5.8S rRNA sequences on the large HindIII fragment (6500 bp) and the presence of the 5S rRNA sequence on the small HindIII fragment. The 3.1 µm molecule from T. glabrata is approximately 2000 base pairs longer than the S. cerevisiae molecule and in addition, one of the HindIII sites lies within the region hybridizing to 25, 18 and 5.8S rRNAs. In S. cerevisiae the 4–5 copies of the 2.8 µm circular DNA molecules per cell, which have an extra-nuclear location, do not appear to be essential for cell viability as in one strain they were undetectable.


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