Nucleic Acids Research, 1980, Vol. 8, No. 5 1043-1059
© 1980
Articles |
Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae
Physiologisch-Chemisches Institut, Universität Marburg Lahnberge, 3550 Marburg/Lahn, GFR
Received November 16, 1979. Two overlapping DNA fragments from yeast Saccharomyces cerevisiae containing the actin gene have been inserted into pBR322 and cloned in E.coli. Clones were identified by hybridization to complementary RNA from a plasmid containing a copy of Dictyostelium actin mRNA. One recombinant plasmid obtained (pYA102) contains a 3.93-kb HindIII fragment, the other (pYA208) a 5.1-kb PstI fragment, both share a common 2.2-kb fragment harboring part of the actin gene. Cloned yeast actin DNA was identified by R-loop formation and translation of the hybridized actin mRNA and by DNA sequence analysis. Cytoplasmic actin mRNA has been estimated to be about 1250 nucleotides long. There is only one type of the actin gene in S.cerevisiae.
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