Skip Navigation

This Article
Right arrow Print PDF (325K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Grzescowiak, K.
Right arrow Articles by Wiewiórowski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grzescowiak, K.
Right arrow Articles by Wiewiórowski, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 5 1097-1105
© 1980

Articles

Chromatography on Sephadex LH20 as an efficient purification step after removal of inter-nucleotide 2,2,2-trichloroethyl protective groups from oligoribonucleotide phosphotriesters1

K. Grzescowiak, R.W. Adamiak and M. Wiewiórowski

Department of Stereochemistry of Natural Products, Institute of Organic Chemistry Polish Academy of Sciences, Noskowskiego 12, 61-704 Poznan, Poland

Received December 19, 1979. Chromatography on Sephadex LH20, in a linear gradient of methanol in 0.02M TEAB buffer pH 7.5, is proposed as a fast and efficient method for the isolation and purification of protected oligoribonucleotide phosphodiesters obtained by deprotection of internucleotide phosphotriesters, and for the monitoring of the deprotection step itself. Its utility is shown on the example of removal of 2,2,2-trichloroethyl groups from oligoribonucleotide phosphotriester I of sequence CCCAUAA by two methods: /1/ reductive elimination with zinc in the presence of acety1acetone2 modified as presented here, and /2/ hydrogenolytic deha1ogenation over palladium in pyridine 3. This method of chromatography on Sephadex LH20 is used as a key purification step during the removal of 2,2,2-trichloroethyl groups from I by method /1/ and allows to raise the yield of III during final deprotection step from 5 to 65%.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.